FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101433
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196461.783676356165105No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT15140.13745729427028244No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC13110.11902675877697509No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA12790.11612145268936012No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC12440.11294377415603128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4550.047.9535373
TTCCGTT4650.045.4326822
CCGTTCT5750.039.145114
GCGAGAC8450.038.5074466
GTTCCGT2000.038.4048081
CTTCCCA7450.036.10532420
TTCCCAT7000.035.96314621
CTTCCGT4650.034.538051
TCGGTAG4300.033.0222663
CCCATCT7900.032.30260523
TCCCATC8100.031.93075222
TCTTCCC7900.031.42956419
GTCTCGC5100.029.8795432
AGACGTC6650.029.0401819
GTTCCGA6650.028.8757951
CGTTCTG3900.028.4065425
CGGGGGC10350.028.3355641
CGAGACG9850.028.1104567
TATAAGC2350.028.0012383
ATCGGTA4600.027.8570652
Produced by FastQC (version 0.11.2)