FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62350
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3510.5629510825982358No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT1070.17161186848436247No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA990.15878107457898957No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC890.14274258219727345No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC750.12028869286287089No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC740.11868484362469929No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.11387329591018443No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG670.107457898957498No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG150.00234995268.9638855
TTCCCAT208.473196E-568.9638821
CTTCCCA252.554926E-455.17111220
CCGGCTG200.007341766351.7229126
AGGGATT404.9628325E-543.1024315
GCACTCG404.9628325E-543.1024321
CTCGTCT350.001341485939.40793224
GCATGTT459.937666E-538.3132720
CCCATCT501.8470139E-434.48194523
TTGATCC709.580508E-734.4819426
GGTCCTT400.002584236734.4819465
TCGTCTC400.002584236734.4819425
ACTCGTC400.002584236734.4819423
CAGTAGT709.580508E-734.4819436
GGGGGGC958.560164E-932.66710319
GGTTGAT751.6426493E-632.18314424
GGATTCT751.6426493E-632.18314417
CATGTTA553.23198E-431.34722121
GGGGCGG1001.4109901E-831.0337521
TGATCCT802.717854E-630.171727
Produced by FastQC (version 0.11.2)