FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752236
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165020.9417681179932383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4950.028.6940163
AGTAGAG8350.028.6162855
GTTCCGA7850.028.4742531
CCGTTCT5500.026.4506874
AGAGTAG6450.026.3177643
GTAGAGA8950.026.3086856
GCGAGAC7700.026.0825796
TCCTACA3550.025.3743572
GTTCTCA7250.024.5682471
GTCTCGC4800.023.8189562
CTTCCGT4650.023.2836341
GTCCTAC1953.274181E-1123.2836321
GACGTCT5350.023.2051320
TGAACCG3500.022.7567625
AGACGTC5700.022.38526219
GGGGGGC30850.022.02147319
TAGGACG2550.021.7285355
CTTCCCA6550.021.5862820
CGGGGGC9900.021.519721
GAGTAGA7850.021.1798044
Produced by FastQC (version 0.11.2)