FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349337
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595434.412759747935468No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25170.18653605437337004No Hit
CGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15650.11598288641014069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCA7650.038.31779520
TCCGTTC4350.037.419033
TCTTCCC7600.036.7548319
GCGAGAC8700.035.41156
GTTCCGA6700.034.4130941
TTCCCAT8450.033.87257821
CCGTTCT4900.032.5085144
GGCCGGG10050.031.0026285
TCCCATC8900.030.99751322
AGACGTC5850.030.06472819
CGTTCTG3950.029.7991525
GACGTCT6300.028.46464320
CTTCCGT4350.028.1082211
CGGGGCG9000.027.9678388
TTCCGTT5900.027.5896342
ACTCGTC4650.027.43946623
CCCATCT9750.027.23409823
GCCGGGG11250.027.0771986
CGGGGGC27850.026.843751
TCGGTAG3550.026.3402143
Produced by FastQC (version 0.11.2)