FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473852
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74190.5033748300372086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC5650.044.732173
TTCCGTT6400.038.950492
CCGTTCT6400.037.318644
GCGAGAC12800.035.9567156
GTTCCGT3050.035.4707761
TCCCATC8950.035.06664722
GTTCCGA8900.034.1144261
GTCTCGC7150.032.927842
CTTCCGT5750.032.77441
TTCCCAT10050.032.6011921
TCTTCCC9550.032.5023719
CTTCCCA9750.032.18938420
TCGTCTC6300.030.66071725
GACCACA8050.028.70579123
AGACCAC9300.027.81340622
ACTCGTC7350.027.68568623
TCCTACA2300.027.0959912
TCGGTAG5250.027.0377253
CCCATCT11750.026.41771523
GAAGACC9400.026.41681920
Produced by FastQC (version 0.11.2)