FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834125
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95510.5207387718939549No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT22930.12501874190690385No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA20740.11307844339944115No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC20550.11204252709057452No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC19670.10724459892319226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACA2900.034.6318742
TCCGTTC5200.034.628083
AGTAGAG9500.034.6214375
TTCCCAT6900.033.4867221
GTCCTAC1500.032.5883331
CTTCCCA7150.032.31585720
GTAGAGA10100.031.185886
AGAGTAG7250.030.0906073
AAGCTTG2800.029.6682036
TCCCATC7700.028.6639622
TTCCGTT6050.028.6213862
GTTCCGA7250.028.414461
GCGAGAC7350.028.255436
CCCATCT8650.027.11063823
CCGTTCT6700.026.8711074
GTTCTCA7000.026.4364531
TCTTCCC8000.026.29582619
TCACTCT7450.026.0241685
CTTCCGT5700.025.7276331
GAGTAGA8800.025.1799184
Produced by FastQC (version 0.11.2)