FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851830
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47820.16768180431512397No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT38350.13447505636731502No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA30520.10701900183391017No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG30140.10568652409154823No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC29980.10512548083160637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC12350.045.1437573
CCGTTCT13450.040.675014
GTTCCGT5800.037.3375661
CTTCCGT12550.036.7377471
CTTCCCA20100.036.54302620
TTCCGTT15500.036.421342
ACTCGTC9850.036.40914523
GTTCCGA19550.035.5540121
TTCCCAT20300.035.33363321
GCGAGAC23250.034.8381966
TCCCATC20950.033.74355322
CGTTCTG9900.033.5712665
AGACCAC16000.032.54452522
AGACGTC19100.032.4982619
TCGTCTC10250.032.30596525
GACGTCT19400.031.64020320
CCCATCT21250.031.15700123
TCTTCCC21850.030.45978519
CGTTCTA4150.030.0321245
ATCGGTA9850.029.5354162
Produced by FastQC (version 0.11.2)