FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456065
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30140.20699625360131585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19670.1350901230370897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC7650.054.310223
TTCCGTT7950.052.2661862
CTTCCGT7350.046.5670361
CCGTTCT8800.046.02464
GTTCCGT3500.041.9103361
CGTTCTG6500.040.470835
GCGAGAC16950.040.0164346
TTCCCAT12550.039.02124821
GCACTCG8350.037.99775321
TCCCATC13100.036.32991422
GTCGTGT4700.035.3423466
CTTCCCA14200.034.7299620
TCGTCTC8150.034.70463625
AGACGTC13450.033.84606619
GACCACA9900.033.44317623
AGTCGTG4900.033.2004245
GTTCCGA13200.033.073181
ACTCGTC9050.032.7733923
AGACCAC10850.031.7853422
CCCATCT14150.031.4416123
Produced by FastQC (version 0.11.2)