FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5997159
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112490.18757214874576444No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT82870.13818209588907013No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA81950.13664803617846383No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC71490.11920644425135302No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG71370.11900634950649132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC24750.039.726733
TTCCGTT25600.037.3276522
CTTCCGT23950.035.275011
GCGAGAC33850.034.0442166
GTTCCGA33950.033.933721
CCGTTCT28400.033.15374
TCCCATC30550.029.01118922
CTTCCCA30250.028.95689220
CGTTCTG17250.027.8890955
TTCCCAT31500.027.69832621
GACGTCT26600.027.48513220
TCTTCCC29950.027.2894719
AGACGTC27000.027.20567119
CTACTAA18500.026.2301251
GTTCCGT12850.025.8093911
ACTCGTC19700.025.73282423
TCATCCG39950.025.383975
TCCTACA12550.025.104942
GTCTCGC22100.024.5962492
TCGTCTC19500.024.40918525
Produced by FastQC (version 0.11.2)