FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110706
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26660.24002751403161593No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT13680.12316490592470014No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA11260.10137696204035992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT4750.043.3595851
TCCGTTC5450.039.3975563
CCGTTCT5650.037.3849224
CTTCCCA6350.034.2165920
TTCCGTT6200.034.076232
CTCCTAG1350.033.6152461
GCGAGAC7750.033.043786
GTTCCGA6800.032.3413661
CGTTCTG4850.030.69335
TCATCCG7300.028.928365
TTCCCAT6950.028.78147521
ACTCGTC4350.028.54191623
TCGTCTC4300.028.07427825
GTCTGCT6850.027.69121423
TCTTCCC7400.027.0312519
CACTAGG658.0822175E-426.8524171
CATCCGA8100.026.4890546
TCCCATC7450.026.38690422
GACGTCT8150.026.23638320
AGACGTC8150.026.23638319
Produced by FastQC (version 0.11.2)