FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640812
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40110.6259246081534053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC2900.048.9779853
TTCCGTT2950.048.151632
CCGTTCT3000.045.0252534
GTTCCGT1400.044.893311
GCGAGAC5900.037.5463946
CTTCCGT2750.036.821581
CGTTCTG1700.036.6635175
AGACGTC5100.035.84116719
CCTAGGT400.00254336634.6429633
TCCCATC4300.034.4886722
GTCTCGC3250.034.1126752
GTCTGCT4900.033.7848223
TTCCCAT4750.032.67347721
AGTCGTG1305.456968E-1231.9630685
ATAGTTG1207.4578566E-1131.7286556
GCACTCG3100.031.15105621
CTTCCCA5400.030.65659720
GACCACA3500.030.5471123
GACGTCT5650.030.52094520
AGACCAC3750.030.3500322
Produced by FastQC (version 0.11.2)