FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909665
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210922.318655768881951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4350.052.528423
TTCCGTT4450.051.3565142
CGTTCTG2400.046.1536875
CCGTTCT4900.044.5127224
GTTCCGT2000.041.9063221
CTTCCGT4550.039.9107861
GCGAGAC6550.039.1008456
GTTCCGA7500.038.6469461
CACTCGT3100.037.8251622
TTGCGTT1350.033.344272
AGTCGTG2050.032.0824435
GACCACA3650.031.18061323
TCCCATC6250.030.34912922
CGTTCTC2300.030.1002315
TTCCCAT6150.029.72106221
GTCGTGT2100.029.665316
TTCCGTA1650.029.3802642
CCCATCT6150.028.59951223
CTCGTCT4000.028.45543924
TGCGAAC1103.5390258E-828.3262773
Produced by FastQC (version 0.11.2)