FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206497
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG465203.8557907727909813No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88930.7370925911958339No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20420.1692503172407391No Hit
CGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12570.10418592006445106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT6850.050.0580444
TTCCGTT6600.049.8758162
TCCGTTC6600.049.871663
GTTCCGT3000.045.426451
CTTCCGT5750.044.3629571
TCGTCTC6850.043.30787725
TTCCCAT13300.042.26908521
CGTTCTC3050.042.0105865
TCCCATC14050.041.48559622
GCGAGAC17700.040.4914636
GTCTCGC7450.039.9993062
AGTCGTG4500.039.247735
GTCGTGT4250.039.1037756
CGTTCTA1950.039.0701375
GACCACA10600.039.04471623
ACTCGTC8350.038.82650423
CGTTCTG4200.038.7530065
GCACTCG8650.038.67608621
GTTCCGA10500.038.6041641
CTTCCCA14700.038.00883520
Produced by FastQC (version 0.11.2)