FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917523
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252812.755353271798091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29810.324896487608485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21130.2302939544839748No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11130.12130486102255747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4700.047.160423
CCGTTCT5050.044.5679174
CTTCCGT4450.043.9618531
GTTCCGA7200.043.667471
TCCCATC8150.041.89329522
TTCCGTT5300.041.823792
GCGAGAC9900.040.9124876
GTCGTGT2400.040.3879666
ATCGGTA3100.040.2216762
GTTCCGT2350.040.1369131
CTTCCCA8350.040.06380520
TTCCCAT8200.039.9555121
AGTCGTG2400.038.9476745
TCGGTAG3350.037.2180183
TCTTCCC8550.035.899719
GTCTCGC4900.035.3421752
TCCGTAC1201.8189894E-1234.6334343
GTCTGCT7350.034.25330423
CGTTCTG3150.032.9715735
CCCATCT10750.032.0817823
Produced by FastQC (version 0.11.2)