FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610575
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24940.15485152818092918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC6750.041.0211833
CCGTTCT6850.039.4043964
CTTCCGT5500.039.333281
GCGAGAC12700.034.8689276
TTCCCAT11200.034.48576721
TTCCGTT8300.033.362682
CTTCCCA11600.033.29556720
TCCCATC12300.030.56055622
CCCATCT12600.030.38031623
TCGTCTC5650.029.91079925
TCTTCCC12400.029.75695219
GACGTCT11200.029.55831120
AGACGTC11300.029.29673419
GAAGACC10150.028.53905720
GTTCCGA10100.028.3285031
GTCTGCT10450.028.05062323
ACTCGTC6350.027.6972323
CGTTCTG5050.027.4065975
GTCTCGC7450.026.9476432
AGACCAC10650.026.87622822
Produced by FastQC (version 0.11.2)