FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822353
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG194341.066423464608668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC7750.032.1548046
GTTCCGA6600.029.6226231
GTCTCGC5700.026.7283742
TCCCATC8300.024.93343422
CTTCCCA8500.024.75254220
AGTAGAG8150.024.6332345
CGAGACG9150.024.5877827
TCTTCCC8000.024.5743419
CTTCCGT5500.024.1212791
TCCGTTC6100.023.837153
CCGTTCT6000.023.6567734
GGGGGGC33450.023.50908519
CGGGGGC11350.022.1470581
GTTCTCA5600.021.19681
CATCCGA9800.021.1904546
GTAGAGA9550.020.657926
AGACGTC5350.020.62971319
CCCATCT9400.020.54741523
TCATCCG10150.020.4614415
GGGGGCG41800.020.05113420
Produced by FastQC (version 0.11.2)