FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622250
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14320.23013258336681397No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT8370.13451185214945763No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC7000.1124949779027722No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA6510.10462032944957814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTT3300.049.3188062
CTTCCGT2550.046.5505451
TCCGTTC3650.044.5824053
GCGAGAC5850.043.774786
CCGTTCT3850.042.2596134
GACCACA2400.040.2337223
GTTCCGT1850.037.7436831
TTCCCAT5050.036.19327521
GTCTCGC3350.035.1449362
CGTTCTC1500.034.611335
CTTCCCA5300.034.48604220
GTCTGCT4800.033.04912623
GTTCCGA5250.032.5853771
CGAGACG6950.031.369237
TCCCATC5400.031.29289222
TCTTCCC5300.031.23264119
ACACCGT450.00455240130.7606566
CCCATCT5100.030.42886423
TCGGTAG2550.029.87043
AGACCAC3500.029.55946522
Produced by FastQC (version 0.11.2)