FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730422
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56750.20784332971240344No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT28950.10602756643478554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT10500.032.9741444
TCCGTTC11800.030.2255363
CTTCCGT9050.029.706871
AGTAGAG16700.029.6405685
GTTCCGA13250.029.5132371
AGAGTAG12150.028.7848433
AGACGTC9250.027.96399519
GTAGAGA17850.027.9187096
TTCCGTT12600.027.7583482
CTTCCCA11350.025.22097820
GTTCTCA13000.024.9778081
GACGTCT10650.024.61181620
GAGTAGA15200.024.3726634
GCGAGAC11200.024.1016986
AAGAGTA12650.023.8161472
GGTCCAA15350.023.58485611
CTAGCAC4300.023.3533043
CGGTCCA16200.022.98713910
ACGGTCC15900.022.553429
CATCCGA14600.022.5186236
Produced by FastQC (version 0.11.2)