FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040247
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28540.2743579169178089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT2750.040.599941
TCCGTTC4200.034.6126373
CCGTTCT4350.034.204884
TTCCGTT4400.033.0409282
GCGAGAC7500.031.363956
GTTCCGA8550.031.0138441
TCGTCTC3750.030.35214825
GTTCCGT2200.030.132771
TCCCATC6650.030.08244322
TTCCCAT6900.029.99223721
CTTCCCA6650.028.52645520
ACTCGTC4350.027.7514423
AGACGTC5650.026.86030619
CCCATCT6350.025.52882823
TCTTCCC6450.025.13303219
GTCTCGC4000.024.2300172
CTCGTCT4700.024.21946724
GACGTCT6200.023.9212320
TCGGTAG2650.023.510473
GCACTCG5450.022.78309621
Produced by FastQC (version 0.11.2)