FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739853
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72280.9769508267182805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT3100.049.55961
TCCGTTC4150.047.5607153
TTCCGTT4100.046.457892
GTTCCGT2050.042.5816961
CCGTTCT4400.042.494554
TCGTCTC3400.039.5692625
TTCCCAT6600.038.66194521
GCGAGAC8700.036.2122276
ACTCGTC4150.034.8977223
CTTCCCA7600.034.0285620
GTTCCGA6300.033.8085141
GACCACA5450.033.5332223
TGCGAAC1253.6379788E-1233.242443
TCCCATC7900.033.17281722
GTCTCGC5000.032.5543142
CTCGTCT4500.032.19652624
GTCTGCT5800.032.10418323
ATCGGTA2700.032.066892
CCCATCT7800.031.82978823
CGTTCTA1850.031.8155775
Produced by FastQC (version 0.11.2)