FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335036
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268302.0096836339993827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18480.13842323353078118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT8300.053.412454
TCCGTTC8700.052.955183
TTCCGTT9050.050.9110262
ATCGGTA5300.045.7541962
CTTCCGT8100.045.696061
GTTCCGT4300.045.47551
GCGAGAC17000.039.9196746
TCCGTAC2300.039.1580963
GTTCCGA12900.037.6255651
GACCACA10200.037.52669523
GTCTGCT12400.036.9868623
TTCCCAT12700.036.92773421
CGTTCTG6050.036.6327745
TTCCGTA2500.036.0281642
TCCCATC13250.035.6551422
TCGTCTC7700.035.38763825
TCGGTAG7200.034.6398543
CTTCCCA14250.034.3629920
GCACTCG8550.034.28232621
CGTTCTC3500.033.6400075
Produced by FastQC (version 0.11.2)