FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627397
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109520.6729765386073588No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT23380.14366500614170974No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA22260.13678285015887334No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC20910.12848739428670447No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC19810.12172813394641872No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG19710.12111365573366548No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC16840.1034781310276472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC5300.041.1700363
TTCCGTT6100.035.7717932
GCGAGAC9700.033.897946
CTTCCCA7450.033.79157620
CCGTTCT6800.032.079494
GTTCCGA8100.031.470111
CTACTAA4900.031.355741
CTTCCGT5950.031.1042211
TTCCCAT8250.030.93282921
CGTTCTG4600.029.350045
TCCCATC8800.028.99952722
GACGTCT8100.028.52539320
AGACGTC8250.028.42476319
CTATTAG2350.028.2322961
TAGGACG1850.028.0686475
GTCCTAC2000.027.9351121
TCTTCCC8850.027.66667419
CTCCTAG1900.027.5675451
TCCTACA4600.027.1065792
GACCACA6750.027.07783923