FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774288
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16110.20806211642179656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4350.052.546613
TTCCGTT4750.048.121632
CCGTTCT5000.044.330234
CTTCCGT4500.043.431561
CGTTCTG2950.039.9134035
TCGTCTC3850.039.40914525
GCACTCG4300.038.49265321
GCGAGAC8150.038.240066
TTCCCAT7250.037.0989521
ACTCGTC4300.036.8887923
TCCCATC7800.036.25135422
CTTCCCA8200.035.3240520
TCTTCCC7400.034.94898619
CTCGTCT4350.034.8816124
TTCCGTA1700.034.6329962
CCGTACT1500.034.632994
TTGCGTT1003.6561687E-1034.632992
GTTCCGT2750.034.265811
TCCGTAC1650.033.5835083
CCCATCT7950.032.5311323
Produced by FastQC (version 0.11.2)