FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309614
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22090.1686756555748488No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA17270.13187091769025072No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT15580.11896635191743521No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC13620.10400010995606339No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG13540.1033892429372319No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC13380.10216750889956887No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC13140.10033490784307436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT4200.034.9185751
GCGAGAC7300.034.614426
CCGTTCT5050.033.5991674
TCCGTTC5250.032.9851043
TTCCGTT5200.032.638732
GTCCTAC1206.730261E-1132.0086941
CTTCCCA7700.031.3497720
GTTCCGA5350.030.676131
TTCCCAT7800.030.5057421
CATGATA3900.030.06362721
GACGTCT5450.029.73914120
TAGGACG2100.029.6763385
TCTTCCC7700.029.55835319
AGACGTC5650.029.29677619
TGCATGA3900.028.2951819
TCCCATC8750.027.587822
CTACTAA3300.027.5116061
CCCATCT8250.026.33380723
CTAATAG1509.058567E-1025.6069561
CTCCTAG2350.025.2602481
Produced by FastQC (version 0.11.2)