FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960766
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48510.5049096241956939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC3200.037.8817753
GCGAGAC4750.036.4404876
GTTCCGA5600.035.5433771
AGTCGTG1950.033.7431645
TCCCATC4900.032.37029622
TTCCGTT3650.032.264212
CTTCCGT3050.030.9126191
CTTCCCA5850.030.65013520
CCGTTCT3850.030.5849484
GACCACA4300.030.47193523
TTCCCAT5550.030.44303921
ATAGGCT802.772651E-630.17122722
GTCGTGT2200.029.8977666
ACTCGTC3250.029.70705423
GTCTCGC4300.028.9980662
CTAAGTT3000.028.8607944
TCTTCCC6050.026.78720719
GAGTCGT1950.026.6407324
GTCTGCT4500.026.05261623
CCACACG5250.025.6187625
Produced by FastQC (version 0.11.2)