FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393332
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39060.9930542137430975No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7780.19779728066874805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.16017003447469313No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6000.15254288997589824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT1950.059.147811
CCGTTCT2500.058.169874
TCCGTTC2650.053.591183
TTCCGTT2600.051.9639432
GTCGTAC200.00737274551.71926549
CGTTCTG1800.050.0073855
TTCCGTA751.6370905E-1146.190172
TTGCGTT751.6370905E-1146.190172
CGTCCAA306.324847E-445.97268335
CGTTCTT1100.044.0624475
TGCGTTC803.274181E-1143.2977523
GTTCCGT1250.041.9411771
GTCGTGT1100.040.9099046
GCGAGAC6350.040.8851286
TCCGTAC856.366463E-1140.7508243
ATCGGTA1800.040.41642
GCGTTCT1050.039.5713354
AGTCGTG1150.039.1362155
ACTCGTC2050.038.69417623
CCCATCT3550.037.88854223
Produced by FastQC (version 0.11.2)