FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686887
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77981.1352667906074798No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA9250.13466552722645792No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT7930.11544839253035799No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC7770.11311904287022465No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG7190.10467515035224136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA2850.034.3072131
CTATAGT1109.295036E-1031.7453081
TAGGACG1251.200533E-1030.4685825
CATGATA1401.4551915E-1129.56252521
GTCCTAC1202.3555913E-929.0998691
GTCGTGT1352.928573E-1028.2116536
CATCCGA3350.027.905426
CTCCTAG906.2816052E-627.1598761
CCTACAT1950.026.6372763
GGTAACG5850.026.5246726
TCCGTTC2350.026.5239263
TCATCCG3400.026.4767115
GGGTTCG5700.026.00899941
TAACGGG5850.025.93523428
ATCCGAT3350.025.8402427
TACAGTG1359.062205E-925.6469575
AGTCGTG1901.8189894E-1225.5119725
AACCGGT951.0524174E-525.41339922
TCCTACA2050.025.3378962
TCAGGGT6000.025.2850138
Produced by FastQC (version 0.11.2)