FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779068
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151511.9447596358726067No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29590.37981280196337164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.10294351712559109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC5450.057.1829643
TCCGTAC1100.056.6631133
GTTCCGT2100.056.596931
CCGTTCT5650.056.384544
TTCCGTT5550.055.528722
CTTCCGT4800.052.4353871
TTCCGTA1200.049.055572
CGTTCTC2550.047.527895
TTGCGTT1350.046.169952
CGTTCTA1150.045.1662525
CACTCGT6050.044.45712322
GTCTCGC6450.044.0225072
GCACTCG6500.043.50134321
TTCCCAT11900.042.01682321
CGTTCTG3550.041.9431235
ATCGGTA4900.041.694292
TCGTCTC6150.040.94656425
TCCCATC13250.039.55759422
GCGAGAC15350.039.4699176
GACCACA9450.039.04147723
Produced by FastQC (version 0.11.2)