FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004459
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72680.7235735853827782No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT12320.12265308987225959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC3500.041.5667953
GCGAGAC8750.039.175866
CCGTTCT4100.034.635534
GACCACA5700.033.27038623
CTTCCCA6700.032.9379720
TTCCCAT6700.032.9379721
AGACCAC6150.031.9588722
TCCCATC7000.031.52634222
ACTCGTC4600.031.48194323
CTTCCGT4350.031.3174271
CCCATCT7300.030.70156723
GACGTCT6450.030.4724120
GTCTGCT6250.030.34259223
TTCCGTT5000.029.7910272
AGACGTC6500.029.70751619
TCTTCCC7150.029.41815819
GTTCCGA5900.028.418421
GCACTCG5150.028.12119321
CGAGACG10900.027.9486437
CCACACG6950.027.29466425
Produced by FastQC (version 0.11.2)