FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919638
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16950.18431165306348804No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11860.12896378792524885No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA9630.10471511616527372No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9440.10264908583594849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4700.050.0973473
TTCCGTT4950.049.665732
CCGTTCT4900.048.052564
CTTCCGT4450.039.2185941
GTTCCGT2550.038.326561
GCACTCG4800.038.07903321
TATAAGC2300.036.131593
AGTCGTG2400.036.059015
GCGAGAC8850.035.1995286
GTTCCGA8200.034.904551
TTCCCAT6650.034.74596421
GTCTCGC5250.034.2963332
CTTCCCA7250.034.24882520
CGTTCTG3750.034.1550945
TCCCATC7200.034.00768722
TCGTCTC4500.033.72029525
ATCGGTA3200.033.544042
AGACGTC7100.033.51521319
GACGTCT7000.033.50133520
GTCGTGT2500.033.2283556
Produced by FastQC (version 0.11.2)