FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760443
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12900.16963796103060977No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11140.14649355704503822No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA9830.1292667563512321No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9070.11927258190291712No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC8460.11125094188519061No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG7900.10388681334432694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAG753.628884E-837.2276461
GCGAGAC4800.036.0477836
TAGGACG905.0258677E-934.6104435
CTTCCCA4700.033.7492120
TCCCATC4900.033.07542422
ATCGGTA2100.032.9710352
GTTCCGA3500.032.906581
TCCGTTC2950.031.6857263
ACTCGTC2450.030.96422623
TTCCCAT5350.030.9379221
CCGTTCT3150.030.764844
TCGGTAG2550.029.867883
TCTTCCC5100.029.7499419
GACGTCT3550.029.1404720
AGTCGTG1452.1827873E-1128.6431265
CCCATCT5100.028.39767323
AGACGTC3950.027.06252119
TTCCGTT3400.026.4738032
GTCTGCT4100.026.0724323
CTAGCAC1607.4578566E-1125.9646933
Produced by FastQC (version 0.11.2)