FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371121
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19350.5213932922146686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.20451550841908703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC2150.059.5803343
TTCCGTT2200.056.6679042
CCGTTCT2350.055.9829034
CGTTCTG1350.051.290385
GTTCCGT1200.043.6307871
CTTCCGT2100.041.5531271
TTGCGTT801.5752448E-938.959182
TGCGTTC801.5788828E-938.9486273
TTCCCAT5000.038.62383321
GCGAGAC5600.037.0889136
CTTCCCA5500.036.366620
CCCATCT4550.035.6224523
GTCTGCT3100.035.59800323
ACTCGTC2250.035.2519123
TCCCATC5500.035.1125822
CGTTCTC1102.7284841E-1134.6210065
CAGTCGT501.8211216E-434.6210064
AGTCGTG1500.034.6210025
GTCGTGT1600.034.6163146
TCTTCCC5250.033.49574719
Produced by FastQC (version 0.11.2)