FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563316
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28550.5068203281994476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC2000.039.8168033
TTCCGTT2250.033.856922
GCGAGAC2600.031.9371856
CCGTTCT2300.031.6069454
CTTCCGT2150.030.8588091
CTTCCCA2550.029.7543220
AGACGTC1850.027.96321119
TAGGTGT756.039415E-527.6788946
ACTCGTC1750.027.59037223
CGCGCTA756.180139E-527.58546816
TCGTCTC1900.027.22733725
CGTTCTG1705.456968E-1226.4648445
GTATTAA1850.026.4253221
GACGTCT1705.456968E-1226.37314820
GTTCCGA2700.025.8660561
AGACCAC2001.8189894E-1224.14157322
ACCGGTA3300.024.03065915
TGAACCG1602.1173037E-923.7929125
TCCCATC2500.023.45181522
CTCGTCT2250.022.99401924
Produced by FastQC (version 0.11.2)