FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAACGATATAGCGATCGCTTGGTTTTAGCCTTAGAAAAGCACATGTCCCATTAGGAACAAGTTTTAGTTGCA14.3478260869565215No Hit
CGGGTGCGGGCGCCGGCGCGGCGGCGGGGGCGGCGGGGGGGGGGGCGGGGCGGGCGCGGTGCCGGCGCGGGGGGG14.3478260869565215No Hit
CGACTAAAACACGTCCGAGGATGCGACATTCCCGATGCGCTTCTCCGACGACACAAACAAAGTCTGGCTTGCTAC14.3478260869565215No Hit
CACCAGTCATACGAGGGGTGTTACCCGCAGCCGAGGGAGCAGATACAAATGAGACGCTGCCGACGAAGAGGAGAG14.3478260869565215No Hit
GAATTTAATCACATATAAGACCTCAACCGGTAAAACGCTTACATAAAAAAAGCAATAGCACAACCAACAATTGTA14.3478260869565215No Hit
TAATTATGTAATTATACCCGTTACTCATAGAGTAAAAGGGTATACTAGACTCGTTAAAAAGTATGTAACACCCGT14.3478260869565215No Hit
GTACGAATGACAGAGCAATTAAAGGTGCTCCCTAATCTGTCAAAAAATCCTAGAATCAGTCACTAGCTGCCCTCA14.3478260869565215No Hit
CTTTGAGGGGGGAGAGTGAGGGGCCGAGGTAGTGCGTTGAGGAAATTAGAGTCGGTAGATCATGATTCAAGTGCC14.3478260869565215No Hit
CTAAAGCGAGACGTGTGCTCTACCCATATCTATATTGAAAGTATCTTATACAAATATGAAGCTGAAGACGATATG14.3478260869565215No Hit
CTAATGCAGGGCAATAAAGGGGTGCCACCGTAGAAAGTAAGTCAATAATGGATAGATTAGAAAAGATATCATTTA14.3478260869565215No Hit
TTTCGCAGCCTACGTACAACGGAGCAGACGCCGATCTGGGGTAGATAATTAGTAAGAAAATGTGCGTGGGTGTTT14.3478260869565215No Hit
CGGTATTGGAGCTGGATTTACCGCGGGTGCTGGCACGAGACTTGCCCTCCAATTGGTCGTTGTTAAAGGATTTAA14.3478260869565215No Hit
ATCCAGCACCGGGCAGAGATCGCCGCCTAGACGTCGACTTTCGTGTTTGCAGAGTGCTGTGTTTTAAATATACAG14.3478260869565215No Hit
CCGCAGAGGGAGCCTGAGAAACGGCTACCACCTCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTC14.3478260869565215No Hit
GTGCTGCCCGGGGGGGGGTGGGCGCGGAGGGGGAGGGAGAGGGGAAGTCCGCCAGGAAGAGTTTGCTGAGGAGCG14.3478260869565215No Hit
GTTCCGTTCTTGCGGGGGAGACGTGTGCTCCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGGAGTGTGT14.3478260869565215No Hit
ACACCGTACTTTTCAGCGATTCTCCACCAGACGGAACGCTCCGGATGAATCGGAGTCCGCTCGTACATGATGGTG14.3478260869565215No Hit
GGGAAGAGCACACGAATCCCCAGTGAGTTCGGATCAGCAAACATCTCACCAAGGAGCTCGGAATCTCTCTCATAT14.3478260869565215No Hit
GCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGC14.3478260869565215No Hit
GTGCACCTATGCCAAGGGAGACGGGTGTGCTGAAGCTCTGAGCACGGGGACACGGGATATAAAAATCTGGAAAGA14.3478260869565215No Hit
GATATCACCAGGCAGACTTGGAGACAACCTGAGTAAAAAGGGACGCCGTTTTTAAAACTATGAATCTCCACACAA14.3478260869565215No Hit
CGGGGACAAGGGGACCGTGGGTAGCGAAACAGGACTGAGATCAGAGGGGCTGTTATATGATTGAGTTGGCTAGAA14.3478260869565215No Hit
CTGCAGGATAAGGGTGTGCAAACGGACAGTACGGCGGACGCCTCCGCCGACGATCTGTCCGGAACGGGCGGGTCG14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)