FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGACGTGTGCTCATCCGAAATACAACCTGTGACATATAATAATCCGAGCTACAACTGCATGCTTATTCTGTTC16.666666666666667No Hit
CTTATAGCTGTTCACTTCCGATATGCTCAGGGAGACGAGTTGAATTAACAACTGCTCAAGGATACAGGGTAACTA16.666666666666667No Hit
ATTTAGCTGTAGTGCATCATGCATTCGCCGCGGGCGTAACAGTTTGCGAACTGGAAGGTGTAGAACCAGTTGATG16.666666666666667No Hit
GGTGGGGATCACGTGACCCAGGACATCGCCCAGCTCCTGAAGATCCCCTTCGAGGAGGCAGAAAGAGTGAAGCGC16.666666666666667No Hit
GGGGTGAAGATGGCCAGGGAGCTCGCGGATGCAGTTCAGGCGGCGTTCCCCGCCTCCGAGGGCGAATGGAGGGTG16.666666666666667No Hit
TGACTAATCAGTTCTTCGAAATTCATACAAACCTTTCTATGTTGGAACGGTGGTTGCCTTCGGAGAAGACAACAC16.666666666666667No Hit
GCTACGCAAGACCAGTGCTCTACCAATCTGAGTGGGAAGACGTGTGCTCTTCATATCTCAAAAGGGAGAAGTAAA16.666666666666667No Hit
CGTCTCCCGGTGCAGATCGGAAGACCACACGTCTCCCGGAGCAGCTCTAAAGAACACAACTGTCGCGGAGGAAAA16.666666666666667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTGAGATACTCCAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAACAAAAAAAAA16.666666666666667No Hit
ATTCCGATCTGGTAGTGGGCCAACTGGAAAAAAAAGAATAAAAATGTTCTAAGGTATATTTCACAGTAACACCAA16.666666666666667No Hit
ATCATGGGGTGAGATTGTCGGGCCTGCGTCGGGGACTTCCCGAGGGCCCTGTTGCGGGTGCGTCAAGCGCCTCGC16.666666666666667No Hit
GTCGCGCACCGACGGCAACATCGTGCGCGCGGCCGAGTAGCTCGGCAACAGCCGCCCGACGCGGTACGACCTGAT16.666666666666667No Hit
CTTCTATTCCGAAGTGTGTAGGGAGACGTGTGCTATTCAGATCTGAATTAGGCGCAGACTGCTACAGATATAAAG16.666666666666667No Hit
GATCGGAAGAGCTCACGTCTCGCTGACCAGATCGGAAGAGCACACCTCTCGCTCAAAAAGTAGAAAGAGATCAGC16.666666666666667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GACCTGCCTGACCGCGTTCTTGGCGAGCTCGGGATGCGATCCACGCCGCGCCAGCAGGCCCGGCTACTCACGCTG16.666666666666667No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)