FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGCCAAGAGGAGCAATAAATCGCAAAGAAAGAGGAGCAAAACGCGCAAATACCGTATGCGCGGTTAAAAAA15.88235294117647No Hit
GTTCTAGATGAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCAAAGAATTTCACC15.88235294117647No Hit
CCGTCCTCTAATCTTATTTTTCGTGACAACCTCCCCGAGCTGCGAGTGGGTAATTTACGCGACTACTGCATTCAT15.88235294117647No Hit
GTGAAAATGCAGGTCGAAAAGCGATTATTATAGTTGATGCCGATAAAACGATCCTAGATCAACTTGTGATTCATA15.88235294117647No Hit
CCATTTGCGCTGGCCATCTTCATCTATGACGAGACCCGTCGTTTCTACTTGCGCCGCAACCACGGAGGATGGTTG15.88235294117647No Hit
ATGCTTTCCTGGTTAAGACGTGCGACAAGCTGGCGAAGATTGTGCAGGACAATTTCGCTGAGGCGGTTGTTTTGC15.88235294117647No Hit
CTTCCCCCCTAAAGAAAAACCAAGACCATTGAGCTCGAAATAGACGCCACGAAATAGACGCTGATGAAGAGAGGG15.88235294117647No Hit
TAGATAGATATTGGGCGTGACACTTTTTGGGAGTTTTATAGGTAGAAGGAGGTGCCTGGTCACCTTGATTTTGGT15.88235294117647No Hit
AAAAAACACCTCTGGTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGATTGGGGGCAT15.88235294117647No Hit
GTTCTCTTCTCTTCTCTTCTCTTCACTTCTCATCAAATCAATTATCATAAAATAAAAAAAAAAATAAAAAAAAAA15.88235294117647No Hit
TTATATGGCTGATCCAGCTGAGTCGCGGGGCATAGCGATGAGCTCGGTGGGTTTTACTTTAAGTAAATGATTTTT15.88235294117647No Hit
CTCTTCACTCTTCTCTTCTCTTCTCTTCTCTTCAGTTCTGTTCTCTTCAGGTGTAAAATAATAACGTATAGAATA15.88235294117647No Hit
GTTCCGATCTGCGCAGGGACCACATCCCTGCGAGTAAAAGACACACACATACTGCGAATTCGAATATCCAGTGAA15.88235294117647No Hit
CATAAAAAAATGCAGAAAAAAAGAAATTAATATAACACTTTCATATAAATATAAATAATCAAATCCAAAAAAGAT15.88235294117647No Hit
CTTCAGTACCTGAGGACCCGGGAGGCGATCGAGAAGGCCCAGAGTTAAAAAAATAAAAAAAAACAATATAAGTAA15.88235294117647No Hit
CTCCAGCAGAGACTCAGTGAAATAGAAATCGCAGTGAAGATGATGTGTACCCTCGGCTAGACGGATAGACCCCGT15.88235294117647No Hit
CCACTACCACAGCAGCAACAAACAGCGCATAAGAACTCGCAGGGCTCCTTCAAAAACATAAAAAAGATTTTCGGC15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)