FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTTTGAAGGTGGTTTCTTTAAAAGTGACTAGATGCTTATCTCTTGATGTTTTTAGAGTTATCTCTATTTAT16.25No Hit
ACCCAGAACCATCGGAAGCTCTCGGTTTTGCCAGAGATGTTCTGAATGTGAATCTTATACGCAGTTATCTATTAA16.25No Hit
CTTCCGATCTGGCACGGGTTGTTCAGTGAGCTCTCAGTGTCCGGAGTTTTTTCTGTTCATATTTGCGGGCGCAGA16.25No Hit
ACATTGCCTCGGCAGGAAGGCGAAACCGGGAACGCATGGCGGAGATAGCGGTGGAGAACCTACTCTTTGCCCTGG16.25No Hit
GTGCACCTTCGGACTGGGCATCTACTCGGGATTGCGGCAGCTGAGTCTGCAGTACAGCAGTTGGGTGAACGCCAT16.25No Hit
CAAATAAGTTCTCCTCCGGTAATAGGGTTACATACATAAAGGTAATGTAATAACCACTAATGTAAGTTGTACTAC16.25No Hit
CTGCTCTACCGAACTCAGTGGGGACACGAGAGCTCATCCAAAATCAGTGGCGCAAGGGATGCAGTTACAATATCA16.25No Hit
GTATGATCTGCTGGATAATGGCTGAACGCCTCTAAGCGAGAGTCCTGTCTGTTATAGACATCTGACGCTGGCGGC16.25No Hit
CGTCTCGGAAGATAACAAGTATCGGAAGCTAACACGTCACGAAATATAACACATAAAGCAAGAGCAAACGTAACA16.25No Hit
NAAGACNCCAAACCAAAAAAGCACAAACTGTACAAATCCAAAATAGCAAAATAATAAAAAAAAATATAACAAGGG16.25No Hit
TCTCCCAAAATAGATCGGAAGAGCACACGTCTCACTATATATATCACAAAAGCACAACTCACCCTAACTAGGAAA16.25Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CGGAGACGTGTTGTCTACCGATCTGACACGGAGACGTGCTGTCTATTATAAACAACTGACGCTGCCGATGATATG16.25No Hit
AACTAAAACAAGTAAATAATCACATATAAGAAAACAACCGGGAAAACGCTTTCATACACCACTCGCTGTCTCTTA16.25No Hit
CTTCCGTGCTTTTATGATATTTGTAGTGTTGGTTTTTTATTTAAAGAGTATTTAAATGGTAGGGATATTTATTGT16.25No Hit
GTGATCAAGGCCTAGGAACACGCAGTCGGCGTTGTGCTGAACATTCGCCTGCGTAATTAGATGCTAGCCGACGTC16.25No Hit
GCTCACGAAAGCTCTACAGATACTGATCTGTATAGCTAACATGCACATAGAAAGCTCGTCATAGAACTACTGAGC16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers