FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTGAGCTGTACCGATGAACGGAGGACACCTGTGCTGATATCTACTAATCTGGAGAGGAGTTGGTTATTAAAT17.6923076923076925No Hit
AAAAACACTATAAATCCTAAAAAAAAAAACAATTGGAGGCAAATAAACGTGACATAAAAAAAAATAATTACAGCT17.6923076923076925No Hit
GTGTGGCCGGTCACCCTCTCAGGCCGGCTACACGTCGTCGCCTTGGTGAGCAATTACCTGACCAACAAGCTGATA17.6923076923076925No Hit
GATCGGAAGAGCACACGTCTCCCGGTGGAGATCGGAAGAGCACACGTCTCGCGGTGGAGATCGGAAGAGCACACG17.6923076923076925Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTACTAATACTTTGAACAAATTAGAGTGCTTAAAGAAGGCTTCAAATGCATTAATATTCTGTGAATGTGATAATG17.6923076923076925No Hit
TTAGGAAAGAGTACAAATAAAATCCTTTAAAAAGGACCAATTGGAGGGCAAGTATGGTGCAAGAATCCGCGGTAA17.6923076923076925No Hit
GCCACGAGAAGACAAATAAAAAGAAGCCAGAAAGCCAGAGACTGCAAGCCATCGCATAAGTCTCTTATACACAAC17.6923076923076925No Hit
CCAAGCCCAGCTCGGAAGAGCAAAGGTCTCCCTCGGCAGAACGGAAGAGCACACGACTCCCTCCGCAGATCAGAA17.6923076923076925No Hit
ACGTAATACTAAACCCCACAAACTCCTTCTATAAATAATTACAACTTGATCTCAAAACCAATTAAAACAAAACAG17.6923076923076925No Hit
CAATAGTCCTCTTCAGATCTGATACGATCAGAAAAGTGAAATAAAGAAATCATACGATCAACACAGTTAAAAAAA17.6923076923076925No Hit
CTGCTCGTGGGTGTGGAACTGGACAGGTAATTCAGCAGCGACTACTAGCTCATCTCCACTTCGGAGGCCAGGACG17.6923076923076925No Hit
CTGATACGTAGCCGACTTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACGCCTACGGGAGGGC17.6923076923076925No Hit
CTTTCACGCCTACCCACAGCTCATCCCCGCAGTTTTTAACCTACGTGGGTTCGAGCGGACACAACCTCTTACAGC17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)