FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATGAATTACATTGTTTTAGATTGATTGGCTTAGAATAAGATCAGTGTCTTATTCAAATCTGGCACTGCCGAA15.88235294117647No Hit
CGAGTCCGCTTGCGATGGGTGTGGGGTAGACGTGTGCTCTTCCGATCTGAGTAGTGTAGAATTGTGATATTACGA15.88235294117647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACATTTGGGGTGTTAGAGCTTTCATGAAGTGATGTACTAGGAATTCAGTTGATTAAATAAGTAGCAGTTAATAC15.88235294117647No Hit
ACTGAACTCCACTTCATTCATATACCTTACATTCCAATCTGTACCATTCAATTAAATTAAAATCAACTCAAGAAA15.88235294117647No Hit
GTACCCGACCGGGCTATCCAAAGATTGTTGTTCACCGACGCCACCAGGACACTGCGTTTCAGCCTAAGGAAGGTA15.88235294117647No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAATAGATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15.88235294117647No Hit
CGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15.88235294117647No Hit
CCTGAAACTAGCACAACATAGTATATTAACCATACTTCAATAAAATTTTTTTAATGCTTAGGTTATTGATTCTGC15.88235294117647No Hit
CCTATGATGAGGAAGCCGATTATCAGGCTCCATATCACGAATCTAACCCTGATTCCCATTTAAAAGTTGCAACCA15.88235294117647No Hit
AGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAAGAGAGAATAGAGAAAAGAGAAACGAGAAGAAAGA15.88235294117647No Hit
CAAATCGACCCGCTGGCGCGATGGCATCCCACAGAACCACCCATTGTATCACCTGTGTAGAACCAACAATAAGCG15.88235294117647No Hit
GGTCAGGGACGTCAATGCACATAATGCATGCACGTCCCTGCTCCCGGATCTGTTACCCGTGCACGAGAAGACGCG15.88235294117647No Hit
AAGTAATAGCAATTGTTGCTATTGGTAATTAGGTGCAAAACAGTTATTCCTGTACTTATCTGACTATTTTTAGAC15.88235294117647No Hit
AGCCAGTTGCTGCCGCTGGGCGGGGTGGAGGGGATTGCGCACGATGCCAGGGAGGGCCTAGAGCGGTCTTCCTGG15.88235294117647No Hit
GTCGGAACTTACCCGACAAGGAAATTCGCTACGTTAGGAGCGTTATTGTAACGGCCGCCGTTTAATGGGACTACA15.88235294117647No Hit
TTCCTGCACCGTCAGCGGCAACGGGTCATCGCCGATGTAGGCCCGGTGGTGGCGCGTGTCGAGGGTCCCCCAACC15.88235294117647No Hit
ATTTAAGGTTAATCCAGTAAATAACGGGTATCAGTGAATAAAACCTGCTATAATAGCAAATACAGCTCCTATAGA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)