FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGATGGCATCGAAAATGGTAAGAACTAGGCCGCTAGCTGATCGCCTTCGAGCCTGAACCTGTCGTGAATG18.333333333333332No Hit
GATCGGATGAGCACACGTCTGCCCAGCCAGATCGGTAGAGCACACGTCTCACCCGACCTCTCTCTGATACCAATA18.333333333333332Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAGATAACAAGCAGATCGTATGGACTTGTACCGCCGACAGATCTTTCAAATGTCTTACCTATCAACTATTGATG18.333333333333332No Hit
CAAATGCAGCTTATTAAAAAGGAGCAACATATGAAAAAATACAAGTATTTAATCACATATAAGAACTCCACCGGT18.333333333333332No Hit
CCCCTTGAAGATCGGAAGAGCACACGTCTCCCTCGTAAGATCGGAAGCTGTCTCTTATACACATCTGACGCTGCC18.333333333333332Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGCCTAGACCAACAGGTCCGCGTAAACCGCTGCGCCCATTTCATGAATGTGATTCACAACTGACACTGATCACAT18.333333333333332No Hit
CTACAACAACAACAACTACAAGTAAACCACACACAAAAGCATCATCTACTCTCTGAGCAATTGAATACCAAACGC18.333333333333332No Hit
TCTCCCAAGAAAGAGCGGAGGAGCACACGGGTCCCGAGAACGGTCTCTGATACACAGCGGACGCGGCCGACGGGG18.333333333333332No Hit
AACATGATCCTTATGGGACATGGACTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGATTTATTGGTTGAAC18.333333333333332No Hit
TCCGTGCGCAGATCGGAAGAGCACACGTCTCCCTGGGCAGATCGGAAGAGCACACGTCTCCCTGCGCCTGTCTCT18.333333333333332Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATATATGCCTACATAATATACCAACAAAGAAGCGTAAGCCCCAAACTTGTCCTTCCGCATGGTTTCACCTGCATG18.333333333333332No Hit
TTTCTATTTGGGGCCGTTGTCCTGCTGCTCTGCGACAAGGAGTCGGAGGAGATCTACTATAAGGAAAGGAAAATA18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)