FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTAATATTGGTTGAACTCTAGATA16.25No Hit
CGCGCAGCTCGGGGGGGGAGTGCGGAAAGGTAACAATACGGGGCTCATATCCGGGGGGCTGGTTGTGGATTGAGT16.25No Hit
GCTAGAAAATATGCACCTATGGACGAGCGTTAACATTATCGCACCAGAAGCACTAGCATAAAAAGACTATCCATT16.25No Hit
CCACTGCGATGTCCCATTCTGCTCGCTCCATGAGGAATTGTCCAAAGGCTGAAAAGCTGGACCACATTAGAAGGA16.25No Hit
CTATATTAATAACCACAATTGTAACTTGAAATACCCGTATGAAGCACAAGTACTCTCTATTATAAACATCTGACT16.25No Hit
ATTAAGAGCTTAATGTAGATGACGAAGGGAGCCAATTGAATTTCTAGAAAGAAAGGATACTGTCACAATAAACAA16.25No Hit
CTCGTGTATTGTAATTTTTCGTCAGTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTACTGCCTTCCTTA16.25No Hit
TGCATCACCTCCGCCCAGTTGTCGCAGTGAACGCCGTGTCCGCCTCTCTCCGAGACTAGCGGGAAAACTTCGGGC16.25No Hit
CGTCACGCGCGCAACATCGGAACAGCACACGACTCCAGGTCTAGTAAAGAACACACCACGACACGCCGAAAAGAA16.25No Hit
CTTCCGATCTCGCGAGGGAGCCGTGTGGTGTGCCGATCTTTTTTTTCTGTCTATTATACACATATGACGCTGCCG16.25No Hit
AGTATTTTTATATAGTTTTATAAAAAGTATATAAAATATTATATAATTTTAAATTGTATATATATTAAGATAGTT16.25No Hit
ACCTCCTGACGGGGGGAGGATGCTAGGCGGTTGGCGGGCAATTTACATGCTCTTATAGAGAGTGATCAGCTCCGT16.25No Hit
ACCACCGGGTGGCTTAACTGGCCACTGGAACGCCACAGGTATGGCCCTCTAGCAAATTGACTCTAACGGTTATAA16.25No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCTGTCTCTT16.25No Hit
CCACATTGGAGCTCTATGTACCCTCCTGCAACATATGGAAATCATAGAGATCTTCAAACCATACAAACTACTCTT16.25No Hit
GGCCAACACAGACCTGTTATTGCACAATCTCATTATTGCTAGACGCAATTTGTCCATTTATGAAGCTAGTGTCCT16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)