FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465980
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18550.3980857547534229TruSeq Adapter, Index 11 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG10820.23219880681574318TruSeq Adapter, Index 11 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG8360.17940684149534314TruSeq Adapter, Index 11 (96% over 30bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT8250.1770462251598781No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA7880.16910597021331386No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC6850.1470020172539594No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC6350.13627194300184559No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA5300.11373878707240655No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC5250.11266577964719517No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG4990.10708614103609596No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG4720.10129190093995451No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG4680.1004334949997854RNA PCR Primer, Index 11 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG553.2522384E-431.3666612
GGGGGGG8300.029.51172469
GTACTAG500.00772259327.6056231
ACCGGTA2250.026.06638515
CCGGTAA2450.023.93851516
CACCGGT2500.023.45974514
CTAGGGG750.001990138323.0022183
TAATACG2550.022.9997520
TATGCCG8000.022.85643
GCCGTCT8100.022.5738346
ACGCTTA2600.022.55744624
CCGTCTT8350.022.31113447
TTACGAC4200.022.180714
ATGGACC953.059842E-421.7915734
TGTCCTA953.0620632E-421.78923610
GTATGCC8750.021.68547842
TGTAGGG800.002895252521.5645792
AGGTTCG1605.7007128E-821.56226567
GAGGTAA2251.0913936E-1121.46643450
ATGCCGT8550.021.3857344
Produced by FastQC (version 0.11.2)