FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13467
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG660.49008687903764764RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG500.3712779386648845RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG380.28217123338531225RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG320.23761788074552612RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG300.22276676319893074RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG300.22276676319893074RNA PCR Primer, Index 41 (96% over 29bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG290.21534120442563304RNA PCR Primer, Index 41 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG240.17821341055914458RNA PCR Primer, Index 41 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.17821341055914458No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG210.1559367342392515TruSeq Adapter, Index 7 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG200.1485111754659538TruSeq Adapter, Index 7 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG170.12623449914606075Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG170.12623449914606075TruSeq Adapter, Index 7 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG170.12623449914606075TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG150.11138338159946537RNA PCR Primer, Index 7 (96% over 28bp)
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG140.10395782282616767Illumina PCR Primer Index 10 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACA150.002305136569.019
GACAGAC150.002305136569.018
TGCCGAC150.002305136569.045
CAGACAT150.002305136569.020
GCCGACT150.002305136569.046
CTTATAC1550.064.548391
AGAGACA252.476786E-455.215
TTATACA1900.052.657892
GGAGATG200.007202754351.752
AGCTTGA200.007202754351.7552
AGCTCAT200.007202754351.7529
ATAAGAG200.007202754351.7512
GTGTATA200.007202754351.758
GATGTGT200.007202754351.755
TAAGAGA200.007202754351.7513
ATGTGTA200.007202754351.756
GTATAAG200.007202754351.7510
GGGGGCG200.007202754351.7567
TGCTTGT200.007202754351.7545
TCATGTC200.007202754351.7535
Produced by FastQC (version 0.11.2)