FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587353
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11160.19000498848222447No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10960.18659988116175452No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC10300.1753630270042036No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC8530.14522782721804436No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC8020.13654480355084592No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA7910.13467199452458745No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG7880.13416122842651695Illumina PCR Primer Index 7 (96% over 27bp)
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG7370.1254782047593185No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG7080.12054079914463704No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA6550.11151726474539161No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA6400.10896343425503914No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT5880.10011015522181721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG500.00773254127.59952451
TATTAGA951.0497679E-525.4206122
TTCTGAG700.00133320524.6424312
GTCCTAG4650.024.4897041
GTATGCC3900.023.88420342
TATGCTT6100.023.7536875
CCTAGAT4950.023.6965623
TAGAGGT1902.5465852E-1123.60686564
TCCTAGA5350.023.2145542
TAGATAC5650.023.203145
GTTAGAG1803.4197E-1023.0015662
CTCGTAT4250.022.7290239
TGCCGTC3650.022.6845445
GCTTGTC5700.022.394358
CACTCTA4800.022.28086749
ATCCCAT5500.021.9541666
AGATTAA6200.021.70123919
CGTATGC4150.021.61408441
TAAGTAG800.00289805421.5621265
TAACCCT800.00289805421.5621264
Produced by FastQC (version 0.11.2)