FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723162
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT28400.16481329091518965No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA24610.14281884117685975No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC21100.12244931120811624No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG19650.11403454811561536Illumina PCR Primer Index 7 (96% over 27bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC18980.11014634723839083No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC18810.10915978880685624No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG18230.10579388356985588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT8950.026.98268347
CGTATGC8900.025.58448641
GTATGCC9000.025.29947942
GCCGTCT9150.025.26177446
TATGCCG9000.024.91615343
TGCCGTC9850.023.4665245
ACGGGTG6500.022.82724469
GTCTTCT10250.022.55075549
ATGCCGT9950.022.19049844
AAGGGGG13050.021.41770269
CTCGCGT8050.020.99956729
TGCTTGA10250.020.53128455
GCTTGAA10250.020.53128456
TCGCGTC8350.020.2450930
GTCTTAC9950.020.1148071
AGACGGG10150.020.05784867
ACGGTCC8650.019.9417849
GGTCCAA8150.019.89529411
GTGTAGG3300.019.867841
AAAGGGG15000.019.78361168
Produced by FastQC (version 0.11.2)