FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500680
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT128510.1713311326439736No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA126450.1685847149858413No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC100020.13334790978951241No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG86470.11528288101878763No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC79300.10572374771354064No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG78840.1051104699840548No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC78160.10420388551438003No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC75660.10087085437586991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT34650.025.08997246
CCGTCTT35200.024.79594647
ATGCCGT34400.024.6705944
GTATGCC36050.024.30699742
TGCCGTC37000.024.05585745
TATGCCG35650.023.51525143
GCTTGAA37850.021.78487856
ACCGGTA42100.021.14172715
CCGGTAA42950.021.12493916
CGTATGC41650.020.79047841
GTCTTCT42900.020.18455949
TGCTTGA39450.020.11425655
GTCTTAC44800.019.0258221
CTCGTAT39650.018.88088239
TATGCTT64750.018.6485755
CACCGGT49150.018.46014414
GCTTGTC62400.018.1341788
TCTTCTG50900.017.9610250
GTCCTAG45500.017.8988481
ATGCTTG64500.017.8650446
Produced by FastQC (version 0.11.2)