FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345025
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG145221.0796825337819Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG89760.6673481905540789Illumina PCR Primer Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG65640.4880206687608037Illumina PCR Primer Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG37280.27716956933885983Illumina PCR Primer Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG37180.27642608873441016Illumina PCR Primer Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG29790.221482872065575Illumina PCR Primer Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22470.16706009181985465No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG14420.10720990316165127Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTC750.001993566322.9992747
CTTCTGC70350.022.60695651
GTATGCC70400.022.54189942
CCGTCTT69450.022.50252347
ATGCCGT70750.022.3816244
TCTTCTG70500.022.3631250
TCGTATG70050.022.35986340
GCCGTCT70100.022.34308446
TATGCCG71450.022.307243
CGTATGC71200.022.24099241
TTCTGCT66750.022.22401452
CTAATCT66300.022.2187534
TGCCGTC70850.022.20395345
AATCTCG65750.022.09061236
TAATCTC66800.022.05326335
GCTTGAA66300.022.01061256
TGCTTGA65500.021.85808455
GTCTTCT70500.021.67803449
CTCGTAT68900.021.58139239
TTACTCG800.002900220721.5618154
Produced by FastQC (version 0.11.2)