FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011935
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG40303513.381264867933737RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31109810.328841757873262RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG1841176.112914123312755RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1713765.68989702633025RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG1587645.271162890301417Illumina PCR Primer Index 10 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG934133.1014281516699396RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG477971.58692003645497RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG399291.325692619528642Illumina PCR Primer Index 10 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG349681.1609812296746111Illumina PCR Primer Index 10 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG234430.7783368498988191RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG230740.7660855894964532RNA PCR Primer, Index 29 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG197240.6548614096917762RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGG137510.45655035716242215RNA PCR Primer, Index 29 (96% over 27bp)
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG103270.34286928502773134RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG102610.3406780026793407RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG95260.3162750856177175RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG88350.29333302345502144Illumina PCR Primer Index 12 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG84810.28157978176819887Illumina PCR Primer Index 10 (96% over 29bp)
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG83860.2784256632364244Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG71020.23579526118591537RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGG67390.2237432082697668Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG65210.21650533627053706RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGG64650.21464606639917527RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG61570.20442008210668555Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG59840.1986762662540858RNA PCR Primer, Index 29 (96% over 30bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGG59140.19635217891488363Illumina PCR Primer Index 10 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG55070.18283927109980794RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG54840.18207564240264149Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG53070.1761990215592302Illumina PCR Primer Index 12 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTG51990.17261328680731822RNA PCR Primer, Index 29 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGG51820.17204886559636912RNA PCR Primer, Index 29 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTTCTTGAAAAAAAAAAAGGGGGGG45850.15222772071774457Illumina PCR Primer Index 12 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGGGGGGGG45350.15056765833260016RNA PCR Primer, Index 29 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG45270.15030204835097702Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45150.14990363337854237Illumina PCR Primer Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42030.1395448440952411Illumina PCR Primer Index 12 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCCTCTTCTGCTTGAAAAAAAAAAAGGGGGGG41060.13632432306806092Illumina PCR Primer Index 12 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAGGGGGGG40490.13443185194899626Illumina PCR Primer Index 12 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG40330.13390063198575003RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAGGGGGGG39350.13064690971086695RNA PCR Primer, Index 29 (96% over 27bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGGGGGGGGGGG38880.12908645106883115RNA PCR Primer, Index 29 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG37730.12526830758299898RNA PCR Primer, Index 29 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31510.10461713151180221Illumina PCR Primer Index 12 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGGGG31280.10385350281463578RNA PCR Primer, Index 29 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGGGGGGGG31240.10372069782382423RNA PCR Primer, Index 29 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGGTTGAAAAAAAAAAGGGGG30430.10103139675989023Illumina PCR Primer Index 12 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA150.00235614768.9983133
CATATAC11450.065.709071
ATTATAC3950.063.782281
TTTATAC650.063.7150761
TTATAAC1150.063.006822
CTTTACA2600.062.3876761
CGTGTGT900.061.3318346
CTTATAC794150.060.637231
TTTACAC2800.060.381542
ATATACA14000.057.916992
TTTGTAA601.8189894E-1257.4985952
TTAGTGA309.130908E-657.4985954
AAACCCA501.1132215E-955.2197271
AACCCAT501.1168595E-955.205982
TGTAGAA252.5681668E-455.19864755
TACCCAT3950.055.0312772
ATACCCA4050.054.5380061
CATAAAC900.053.6858441
GCTAACA453.031164E-853.66534833
GTGTGTT1250.052.43871347
Produced by FastQC (version 0.11.2)