FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991614
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA67140.33711351697668324No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA47240.23719455677656412No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG36060.18105918114654748No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG36020.18085833901549195No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA34230.17187065365075763No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA31620.15876570459938524No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25620.12863938494105787RNA PCR Primer, Index 29 (96% over 30bp)
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT24050.12075633129712887No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG21960.11026232994947817No Hit
TCTTATATGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATTTAAAAACCTGCATTAGTGCTCTTAAAC20130.10107380245368831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10300.027.13042643
ATGCCGT10350.026.99936344
GTATGCC10750.026.957542
CGTATGC10800.026.83269941
CCGTCTT10450.025.75058747
TGCCGTC11050.025.28899445
GCTTGAA10350.024.66608456
AATCTCG11450.024.10423336
TGCTTGA10300.023.7809955
GCCGTCT11200.023.7181946
GTCTTCT11700.022.99945649
CTCGTAT11950.022.80699239
CTTCTGC13350.021.96577551
GACGGGT10400.021.89756268
TCTTCTG14500.021.6512150
ACGGGTG9750.021.58789869
AGGTTCG6400.021.5657867
AAGGGGG15950.021.20071869
TAATCTC14000.021.19235635
GAGACGG19300.020.9176966
Produced by FastQC (version 0.11.2)