FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2ss86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2ss86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324533
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13280.10026175263281474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5600.025.87409242
GCCGTCT5700.024.81492246
TCGTATG5500.024.46277840
GATCTTT21300.023.4850926
TGCCGTC6100.022.6221645
ACCGTAC5550.022.3775968
GCTTGAA5650.021.98153156
GAGACGG12400.021.70576966
AGACGGG11150.021.66334267
ATCTTTT20200.021.5190497
TAGTCGC2051.7389539E-920.19746461
TTACTCG2402.7284841E-1120.1242964
CGTATGC7050.020.06312841
CCGTCTT6550.020.01456647
TGCTTGA5750.019.79930755
GTTCTAG2102.3519533E-919.71359657
TATGGAC3900.019.4608573
CGATCTT40150.019.4189975
CTCGTAT6600.019.3402339
TATGCCG7200.019.16599543
Produced by FastQC (version 0.11.2)